Genomics: The Mapping And Sequencing Of Genomes

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Question 1

Free

Short Answer

Please select the best match for each term.
A)Eukaryotic cloning vector
B)Molecule used in DNA sequencing
C)Origin of replication
D)Small circular fragment of DNA
E)Endonuclease isolated from bacteria
-1)Restriction enzyme
2)ARS
3)Plasmid
4)YAC
5)Dideoxynucleotides

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Question 2

Free

Multiple Choice

When DNA is cut with a restriction enzyme,the resulting fragments have

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5' carboxyls.

5' hydroxyls.

3' hydroxyls.

3' phosphates.

3' carboxyls.

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Question 3

Free

Multiple Choice

A plasmid used as a cloning vector in E.coli must have

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an ori sequence.

a selectable marker.

unique restriction sites.

B and C

All of these

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Question 4

Free

Multiple Choice

Bacteria protect their own DNA from restriction enzyme damage by adding ________ groups to certain nucleotides.

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methyl

hydroxyl

phosphate

amino

carboxyl

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Question 5

Free

Multiple Choice

A polylinker region in a cloning plasmid is characterized by

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multiple methyl groups.

multiple restriction cut sites.

multiple reporter genes.

multiple expression vectors.

multiple cloning targets.

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Question 6

Multiple Choice

In a physical map,distances are measured in

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recombination frequencies.

number of SNPs.

base pairs.

probabilities.

microns.

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Question 7

Multiple Choice

In DNA electrophoresis,fragment separation is based on

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fragment size.

fragment nucleotide content.

fragment sequence.

fragment charge.

All of these

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Question 8

Multiple Choice

The chemical "label" that permits visualization of DNA fragments in an automated sequencing machine is

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³²P.

³⁵S.

fluorescent dyes.

tritium.

None of these

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Question 9

Multiple Choice

A DNA copy of an mRNA molecule is called

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dDNA.

rDNA.

mDNA.

shDNA.

cDNA.

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Question 10

Multiple Choice

The first complete nonviral genome sequenced was that of

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Escherichia coli.

Methanococcus.

Saccharomyces cerevisiae.

the human mitochondrion.

Mus musculus.

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Question 11

Multiple Choice

Many bacteria are naturally capable of taking up endogenous fragments of DNA in their environment,a phenomenon termed

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conversion.

transformation.

hybridization.

transfection.

conjugation.

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Question 12

Multiple Choice

An SNP always occurs due to

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a one base-pair change.

a three base-pair change.

a single restriction site change.

a single amino acid change.

None of these

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Question 13

Multiple Choice

The recognition sequences for many restriction enzymes are

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terminal repeats.

tandem repeats.

palindromic.

inverted repeats.

dual repeats.

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Question 14

Multiple Choice

In addition to restriction enzymes,which of the following enzymes is required to insert a fragment of DNA into a cloning vector?

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DNA polymerase

DNA ligase

RNA polymerase

Reverse transcriptase

Topoisomerase

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Question 15

Multiple Choice

Which restriction enzyme leaves blunt ends?

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XbaI

SmaI

EcoR1

BamHI

All of these

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Question 16

Multiple Choice

A restriction enzyme cuts DNA and leaves the following end: CTGCA G
Which of the following could be the sequence of the corresponding end of the other fragment generated by the enzyme?

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G CTGCA

C GTGCA

CCGAT G

GGCTA C

None of these

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Question 17

Multiple Choice

The ________ method was used by the Celera Genomics Corporation to sequence the human genome.

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FISH

whole-genome shotgun

clone contig

radiation hybrid

pedigree analysis

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Question 18

Multiple Choice

Candidate open reading frames of a genome are identified by searching for

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a start codon "in frame" with a stop codon.

a start codon.

introns and exons.

a promoter.

All of these

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Question 19

Multiple Choice

Successful insertion of a DNA fragment into the polylinker region of pBluescript II is detected by

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production of the enzyme β-galactosidase.

absence of the enzyme β-galactosidase.

the increased size of the plasmid.

autoradiography.

None of these

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Question 20

Multiple Choice

In gel electrophoresis,which of the following DNA fragments would travel the farthest distance from the sample well?

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ATCCCGAT

ATCCCG

ATCC

ATCCCGATTGCACGTT

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Genomics: The Mapping And Sequencing Of Genomes

Please select the best match for each term.A)Eukaryotic cloning vectorB)Molecule used in DNA sequencingC)Origin of replicationD)Small circular fragment of DNAE)Endonuclease isolated from bacteria-1)Restriction enzyme2)ARS3)Plasmid4)YAC5)Dideoxynucleotides

When DNA is cut with a restriction enzyme,the resulting fragments have

A plasmid used as a cloning vector in E.coli must have

Bacteria protect their own DNA from restriction enzyme damage by adding ________ groups to certain nucleotides.

A polylinker region in a cloning plasmid is characterized by

In a physical map,distances are measured in

In DNA electrophoresis,fragment separation is based on

The chemical "label" that permits visualization of DNA fragments in an automated sequencing machine is

A DNA copy of an mRNA molecule is called

The first complete nonviral genome sequenced was that of

Many bacteria are naturally capable of taking up endogenous fragments of DNA in their environment,a phenomenon termed

An SNP always occurs due to

The recognition sequences for many restriction enzymes are

In addition to restriction enzymes,which of the following enzymes is required to insert a fragment of DNA into a cloning vector?

Which restriction enzyme leaves blunt ends?

A restriction enzyme cuts DNA and leaves the following end: CTGCA GWhich of the following could be the sequence of the corresponding end of the other fragment generated by the enzyme?

The ________ method was used by the Celera Genomics Corporation to sequence the human genome.

Candidate open reading frames of a genome are identified by searching for

Successful insertion of a DNA fragment into the polylinker region of pBluescript II is detected by

In gel electrophoresis,which of the following DNA fragments would travel the farthest distance from the sample well?

Please select the best match for each term.
A)Eukaryotic cloning vector
B)Molecule used in DNA sequencing
C)Origin of replication
D)Small circular fragment of DNA
E)Endonuclease isolated from bacteria
-1)Restriction enzyme
2)ARS
3)Plasmid
4)YAC
5)Dideoxynucleotides

A restriction enzyme cuts DNA and leaves the following end: CTGCA G
Which of the following could be the sequence of the corresponding end of the other fragment generated by the enzyme?