Recombinant Dna Technology

This question bank verified by Studydeets

All Questions

Question 1

Free

Short Answer

Please select the best match for each term.
A)A chloroplast enzyme
B)Repetitive sequence used in DNA fingerprinting
C)Short oligonucleotide probe
D)Identified by mRNA
E)Stems from single base substitution,for example
-1)EST
2)STR
3)SNP
4)EPSPS
5)ASO

Check answer

Question 2

Free

Short Answer

Please select the best match for each term.
A)Technique used to generate copies of a DNA fragment
B)Screening procedure used to assay DNA fragments
C)Movement of macromolecules in an electrical field
D)Screening procedure used to assay mRNA fragments
E)DNA fragments generated by restriction enzymes
-6)PCR
7)Southern blot
8)RFLPs
9)Northern blot
10)Electrophoresis

Check answer

Question 3

Free

Multiple Choice

________ were the first class of DNA sequence polymorphisms discovered in the human genome.

Choose correct answer/s

SNPs

Microsatellites

VNTRs

STRs

RFLPs

Check answer

Question 4

Free

Multiple Choice

RFLPs arise due to

Choose correct answer/s

changes in the number of tandem repeats.

base changes that add or delete restriction enzyme recognition sites.

addition or deletion of bases between restriction enzyme recognition sites.

B and C only

All of these

Check answer

Question 5

Free

Multiple Choice

Which of the following sequences is considered an STR?

Choose correct answer/s

CGTATTATGC

TACGTACGTACG

CAGCAGCAG

TATTACGCCG

A and B only

Check answer

Question 6

Multiple Choice

Expressed sequence tags,or ESTs,

Choose correct answer/s

are genetic markers generated from cDNA.

pertain to expressed genes only.

are oligonucleotides derived from mRNA.

A and C only

All of these

To unlock the question

Question 7

Multiple Choice

Which of the following techniques is best suited to study protein interactions?

Choose correct answer/s

PCR

The yeast two-hybrid system

Restriction mapping

RT-PCR

Southern blotting

To unlock the question

Question 8

Multiple Choice

In yeast,expression of the GAL genes is induced when there is ________ in the culture medium.

Choose correct answer/s

glucose

lactose

galactose

maltose

Any of these

To unlock the question

Question 9

Multiple Choice

When yeast cells are cultured in the presence of both glucose and galactose,the GAL genes are

Choose correct answer/s

transcribed and then translated.

transcribed but not translated.

neither transcribed nor translated.

translated but not transcribed.

transcribed and translated simultaneously.

To unlock the question

Question 10

Multiple Choice

For the yeast galactose metabolizing gene,GAL1,to be transcribed,the regulatory protein produced by the GAL4 gene binds to

Choose correct answer/s

RNA polymerase.

upstream activator sequence G (UAS_G)region.

a ribosome.

the operator sequence.

the repressor protein.

To unlock the question

Question 11

Multiple Choice

ASO probes are used for the molecular testing of

Choose correct answer/s

chromosomal rearrangements.

single nucleotide changes in a DNA region.

RFLP fragments in a DNA region.

defective proteins implicated in disease.

successfully cloned Ti plasmids.

To unlock the question

Question 12

Multiple Choice

Vectors capable of entering two or more different host organisms are known as

Choose correct answer/s

dual vectors.

omnivectors.

shuttle vectors.

parity vectors.

universal vectors.

To unlock the question

Question 13

Multiple Choice

blots are used to study DNA fragments,while blots are used to study RNA fragments.

Choose correct answer/s

Western,northern

Southern,western

Eastern,northern

Southern,northern

Western,Southern

To unlock the question

Question 14

Multiple Choice

In a sequenced genome,candidate protein-encoding genes are identified by searching for

Choose correct answer/s

STRs.

RFLPs.

ESTs.

SNPs.

ORFs.

To unlock the question

Question 15

Multiple Choice

A(n) ________ is a photograph using X-ray film that is used to identify the position of radiolabeled molecules on a gel.

Choose correct answer/s

Southern blot

northern blot

X-rayogram

autoradiogram

radiocopy

To unlock the question

Question 16

Multiple Choice

Which of the following is used to screen newborns for the genetic disease PKU?

Choose correct answer/s

RFLPs

ASOs

Antibodies

PCR

All of these

To unlock the question

Question 17

Multiple Choice

Successful cotransformation in the yeast two-hybrid system will result in the binding of proteins produced by each yeast expression vector.These fusion proteins function by

Choose correct answer/s

binding to the lacZ operator,preventing transcription.

binding to RNA polymerase,preventing transcription.

binding to the lacZ repressor,enabling transcription.

binding to the lacZ upstream activator sequence,enabling transcription.

changing color in the presence of X-gal.

To unlock the question

Question 18

True/False

DNA markers are DNA polymorphisms that are useful for genetic mapping.

Choose correct answer/s

True

False

To unlock the question

Question 19

True/False

Somatic cell gene therapy can result in a cure for a genetic disease in an individual as well as any progeny produced by the individual.

Choose correct answer/s

True

False

To unlock the question

Question 20

True/False

Humulin is insulin created by recombinant DNA technology.

Choose correct answer/s

True

False

To unlock the question

Recombinant Dna Technology

Please select the best match for each term.A)A chloroplast enzymeB)Repetitive sequence used in DNA fingerprintingC)Short oligonucleotide probeD)Identified by mRNAE)Stems from single base substitution,for example-1)EST2)STR3)SNP4)EPSPS5)ASO

________ were the first class of DNA sequence polymorphisms discovered in the human genome.

RFLPs arise due to

Which of the following sequences is considered an STR?

Expressed sequence tags,or ESTs,

Which of the following techniques is best suited to study protein interactions?

In yeast,expression of the GAL genes is induced when there is ________ in the culture medium.

When yeast cells are cultured in the presence of both glucose and galactose,the GAL genes are

For the yeast galactose metabolizing gene,GAL1,to be transcribed,the regulatory protein produced by the GAL4 gene binds to

ASO probes are used for the molecular testing of

Vectors capable of entering two or more different host organisms are known as

________ blots are used to study DNA fragments,while ________ blots are used to study RNA fragments.

In a sequenced genome,candidate protein-encoding genes are identified by searching for

A(n) ________ is a photograph using X-ray film that is used to identify the position of radiolabeled molecules on a gel.

Which of the following is used to screen newborns for the genetic disease PKU?

Successful cotransformation in the yeast two-hybrid system will result in the binding of proteins produced by each yeast expression vector.These fusion proteins function by

DNA markers are DNA polymorphisms that are useful for genetic mapping.

Somatic cell gene therapy can result in a cure for a genetic disease in an individual as well as any progeny produced by the individual.

Humulin is insulin created by recombinant DNA technology.

Please select the best match for each term.
A)A chloroplast enzyme
B)Repetitive sequence used in DNA fingerprinting
C)Short oligonucleotide probe
D)Identified by mRNA
E)Stems from single base substitution,for example
-1)EST
2)STR
3)SNP
4)EPSPS
5)ASO

blots are used to study DNA fragments,while blots are used to study RNA fragments.