Regulation Of Gene Expression In Bacteria And Bacteriophages

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Question 1

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Short Answer

Please select the best match for each term.
A)Molecule capable of down-regulating protein synthesis
B)Regulatory molecule that leads to gene expression
C)Adjacent genes serving one function that are transcribed and regulated together
D)General class of molecules that help control regulation of expression
E)Regulatory region to which repressor protein binds
-1)Operator
2)Repressor protein
3)Inducer
4)Effector molecule
5)Operon

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Question 2

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Multiple Choice

The order of the structural genes in the lac operon is

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5'-lacY-lacZ-lacA-3'.

3'-lacZ-lacY-lacA-5'.

5'-lacZ-lacY-lacA-3'.

3'-lacY-lacA-lacZ-5'.

5'-lacA-lacY-lacZ-3'.

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Question 3

Free

Multiple Choice

In the lac operon in E.coli,a nonsense mutation in the ________ gene will result in a loss of β-galactosidase activity in the cell.

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lacZ

lacI

lacY

Both A and B

All of these

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Question 4

Free

Multiple Choice

The inducer of the lac operon is

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galactose.

glucose.

allolactose.

repressor protein.

permease.

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Question 5

Free

Multiple Choice

Attenuation of the trp operon fails to occur when

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a lack of tryptophan causes stalling of ribosomes on the leader sequence of the RNA.

an RNA stem-loop forms that allows transcription to continue.

an RNA stem-loop that causes transcription to terminate fails to form.

the cell has enough tryptophan.

the operator is mutated.

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Question 6

Multiple Choice

To inhibit the transcription of operon genes,the lacI gene product binds to

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the operator.

the promoter.

the repressor.

the inducer.

β-galactosidase.

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Question 7

Multiple Choice

A lacO^C mutation is

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constitutive.

cis-dominant.

incompletely dominant.

codominant

recessive.

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Question 8

Multiple Choice

During transcription of the trp operon,pairing of regions 2 and 3 in the leader peptide mRNA creates a(n) ________ signal.

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continuous

termination

antitermination

induction

repression

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Question 9

Multiple Choice

What is the underlying reason that a lambda phage would need to switch from the lysogenic state to the lytic state?

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To have its genome replicated

To kill its host cell

To make more of the Cro protein

To be more efficient in its use of resources

To escape an endangered host cell

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Question 10

Multiple Choice

Which of the following mutations is dominant over wild-type lacI⁺ in a partial diploid experimental cell?

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lacA^-

lacO^-

lacI^-

lacI^S

C and Donly

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Question 11

Multiple Choice

Which positive regulatory protein is required for the production of phage coat protein in lambda?

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Cro

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cII

cIII

B and C only

A,B,and C

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Question 19

Multiple Choice

To maintain lysogeny in lambda,which of the following needs to occur?

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Binding of repressor protein to O_L

Binding of repressor protein to O_R

Inactivation of P_L

Inactivation of P_R

All of these

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Question 20

Multiple Choice

The genetic switch for the infection pathways of lambda phage is controlled by two regulatory proteins: and .

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lambda repressor,Cro

lambda repressor,permease

excisionase,integrase

recA,Cro

lyase,lysogenase

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Regulation Of Gene Expression In Bacteria And Bacteriophages

The order of the structural genes in the lac operon is

In the lac operon in E.coli,a nonsense mutation in the ________ gene will result in a loss of β-galactosidase activity in the cell.

The inducer of the lac operon is

Attenuation of the trp operon fails to occur when

To inhibit the transcription of operon genes,the lacI gene product binds to

A lacOC mutation is

During transcription of the trp operon,pairing of regions 2 and 3 in the leader peptide mRNA creates a(n) ________ signal.

What is the underlying reason that a lambda phage would need to switch from the lysogenic state to the lytic state?

Which of the following mutations is dominant over wild-type lacI+ in a partial diploid experimental cell?

Which positive regulatory protein is required for the production of phage coat protein in lambda?

How many structural genes are under the control of the trp operon promoter?

In terms of regulation of gene expression,tryptophan and allolactose are examples of ________ molecules.

Which of the following can be a transdominant mutation in partial diploid cells?

The interaction between allolactose and the repressor protein results in a shape change known as a(n)

Operons can best be defined as

The ________ pathway in lambda phage leads to integration of viral DNA with that of the host,while the ________ pathway leads to induction of progeny viruses.

In bacteriophage lambda,the establishment of lysogeny requires the protein product of which gene?

To maintain lysogeny in lambda,which of the following needs to occur?

The genetic switch for the infection pathways of lambda phage is controlled by two regulatory proteins: ________ and ________ .

A lacO^C mutation is

Which of the following mutations is dominant over wild-type lacI⁺ in a partial diploid experimental cell?

The pathway in lambda phage leads to integration of viral DNA with that of the host,while the pathway leads to induction of progeny viruses.

The genetic switch for the infection pathways of lambda phage is controlled by two regulatory proteins: and .